Lab 3: Chromatagraphy Lab

I.Introduction

The Chromatography lab was a lab on the technique used to identify and analyze components of a mixture.Paper chromatography  is an easy way to separate  the soluble components of a homogeneous mixture.—Separation is based on

  1. Solubility of the sample in the mobile phase.
  2. Molecule size
Purpose: The purpose of this lab was to reproduce multicolor art patterns with a variety of black ink pens using paper chromatography.


II. Lab Questions 

1. Why is it important that only the wick and not the filter paper be in contact with the water in the cup?

Answer: It is important that only the wick and not the filter paper be in contact with the water in the cup because only then will the water be evenly distributed and the ink will dissolve properly. If this was not the case, the filter paper would soak and rip.

2. What are some variables that will affect the patter of the colors produced on the filter paper?

Answer: Some variables that will affect the pattern of the colors produced on the filter paper are ink color and initial design.

3. Why does ink separate into different pigment bands?

Answer: Every color dye has its own properties and therefore they seperate into different pigment bands. In example, water solubility and molecule size are huge factors because if the pigment is not water soluble, the pigment will not travel to far and stay around the center. If the pigment is highly water soluble, then the pigment will travel farther. 

4. Choose one color that is present in more than one type of ink. Is the pigment that gives this color always the same? Do any of the pens appear to contain common pigments? Explain.

Answer: One color that is present in most of the types of ink was blue. The pigment that gives this color is always the same because it is an ingredient in the black ink of the pens. Most of the pens have common pigments because when they were put on a chromatogram  they  produced color like blue, yellow, and pink consistently. Therefore the pigment that gives the blue color is always the same because blue is seen on almost every chromatogram.

5. Why are only water soluble markers or pens used in this activity? How could this experiment be modified to separate the pigments in permanent markers or pens?

 Answer: Only water soluble markers or pens were used in this activity because  only then would the ink dissolve in the solute water and be spread across the filter paper . Permanent markers would not work for this experiment because water does not make permanent ink run through the filtration paper. If we wanted the experiment to work on permanent markers or pens, we could change the solvent from water to another liquid or chemical.


III. Favorite Chromatogram








(Made with black pen)




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